Investigation of Antioxidant Enzymes and Biochemical Changes in the Wheat Seeds (Freed) Induced by Different Pre-Sowing Treatments

In the present work, experiment was conducted using wheat seeds (Freed) to study the effect of different pre-sowing seed treatments on biochemical changes in the seeds. Different chemical and hormonal treatments along with non-treated controls were applied in three combinations i.e. combination A (IAA, IBA NAA and H O 100mM each), combination B (100mM BAP, kinetin, H O and 2.8mM GA3) and combination 2 2 2 2 C (100mM IAA, NAA, kinetin H O and 2.8mM GA3). Results depicted that seed treatment with different 2 2 combinations increased the activity of superoxide dismutase in primed seeds. Highest superoxide dismutase activity was observed in the seed treated with the combination C. Peroxidase activity was found to be highest in hydro primed seeds. Whereas, catalase activity was increased in seeds after all applied treatment combinations with maximum increase in combination C. Results further exhibited that level of total soluble protein and protease activity were raised after all treatment combinations including simple water soaking treatment being highest in combination A. However, highest increase in amylase activity was recorded in the seeds treated with combination A.